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anti ck18 primary antibody  (Bioss)


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    Structured Review

    Bioss anti ck18 primary antibody
    Anti Ck18 Primary Antibody, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+ck18+primary+antibody/CK18+Polyclonal+Antibody/pm41207443-114-10-15
    Average 94 stars, based on 26 article reviews
    anti ck18 primary antibody - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Incubation:

    Article Title: Sinomenine hydrochloride ameliorates fatty acid-induced bovine mammary epithelial cells oxidative stress and inflammation via enhancing autophagy activity.
    Article Snippet: After fixation, cells were permeabilized at room temperature using 0.1% Triton X-100 (T9284; Sigma-Aldrich) for 10 min and subsequently blocked at room temperature with 5% goat serum (C0265; Beyotime) for 1 h to reduce nonspecific binding. .. Cells were then incubated overnight at 4°C with either the anti-CK18 primary antibody (1:100; bs-2043R; Bioss) or an isotype-matched IgG control (1:100; ab37415; Abcam). .. The next day, cells were washed 3 times with PBS and then incubated with a fluorescein isothiocyanate–conjugated goat anti-rabbit IgG secondary antibody (1:500; ab150077; Abcam) for 50 min at room temperature.

    Control:

    Article Title: Sinomenine hydrochloride ameliorates fatty acid-induced bovine mammary epithelial cells oxidative stress and inflammation via enhancing autophagy activity.
    Article Snippet: After fixation, cells were permeabilized at room temperature using 0.1% Triton X-100 (T9284; Sigma-Aldrich) for 10 min and subsequently blocked at room temperature with 5% goat serum (C0265; Beyotime) for 1 h to reduce nonspecific binding. .. Cells were then incubated overnight at 4°C with either the anti-CK18 primary antibody (1:100; bs-2043R; Bioss) or an isotype-matched IgG control (1:100; ab37415; Abcam). .. The next day, cells were washed 3 times with PBS and then incubated with a fluorescein isothiocyanate–conjugated goat anti-rabbit IgG secondary antibody (1:500; ab150077; Abcam) for 50 min at room temperature.



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    Santa Cruz Biotechnology ck18 primary antibodies
    (a) qRT-PCR analysis of TIC-associated genes in HTRZ, HTRY, and HTRY-LT #1 cells. (b) Immunoblot of BMI1, ubiquitinated histone H2A (ubH2A), p16INK4a, and p14ARF, in addition to the TIC markers CD44 and CD49f, in HTRZ and HTRY cells. (c) HTRZ and HTRY cells grown in mammosphere cultures and serially passaged. Doxycycline was replenished at each generation. (d) Differentiation culture of cells from secondary mammospheres. Colonies (x = 50) were evaluated for markers of luminal <t>(CK18)</t> and basal (CK14) differentiation using immunofluorescence. (e) FACS histograms depicting two HTRY cell populations defined as CD44/CD49f double-positive and CD44/CD49f double-negative. (f) Mammosphere assay of sorted HTRY cells. Data represented as mean ± SEM. P values were determined using t test. *, P <0.05; **, P < 0.01.
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    Image Search Results


    (a) qRT-PCR analysis of TIC-associated genes in HTRZ, HTRY, and HTRY-LT #1 cells. (b) Immunoblot of BMI1, ubiquitinated histone H2A (ubH2A), p16INK4a, and p14ARF, in addition to the TIC markers CD44 and CD49f, in HTRZ and HTRY cells. (c) HTRZ and HTRY cells grown in mammosphere cultures and serially passaged. Doxycycline was replenished at each generation. (d) Differentiation culture of cells from secondary mammospheres. Colonies (x = 50) were evaluated for markers of luminal (CK18) and basal (CK14) differentiation using immunofluorescence. (e) FACS histograms depicting two HTRY cell populations defined as CD44/CD49f double-positive and CD44/CD49f double-negative. (f) Mammosphere assay of sorted HTRY cells. Data represented as mean ± SEM. P values were determined using t test. *, P <0.05; **, P < 0.01.

    Journal: Stem cells (Dayton, Ohio)

    Article Title: YB-1 transforms human mammary epithelial cells through chromatin remodeling leading to the development of basal-like breast cancer

    doi: 10.1002/stem.1707

    Figure Lengend Snippet: (a) qRT-PCR analysis of TIC-associated genes in HTRZ, HTRY, and HTRY-LT #1 cells. (b) Immunoblot of BMI1, ubiquitinated histone H2A (ubH2A), p16INK4a, and p14ARF, in addition to the TIC markers CD44 and CD49f, in HTRZ and HTRY cells. (c) HTRZ and HTRY cells grown in mammosphere cultures and serially passaged. Doxycycline was replenished at each generation. (d) Differentiation culture of cells from secondary mammospheres. Colonies (x = 50) were evaluated for markers of luminal (CK18) and basal (CK14) differentiation using immunofluorescence. (e) FACS histograms depicting two HTRY cell populations defined as CD44/CD49f double-positive and CD44/CD49f double-negative. (f) Mammosphere assay of sorted HTRY cells. Data represented as mean ± SEM. P values were determined using t test. *, P <0.05; **, P < 0.01.

    Article Snippet: After 7 days, cells were fixed in 100% methanol at -20°C for 20 minutes and stained with CK14 and CK18 primary antibodies (Santa Cruz Biotechnology, Santa Cruz, CA, USA).

    Techniques: Quantitative RT-PCR, Western Blot, Immunofluorescence